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RADI COVID-19 Detection Kit is used for qualitative detection of 2019 novel coronavirus in
human Nasal Swab or Sputum by using ABI 7500, Bio-Rad CFX 96 Platform, etc.
RADI COVID-19 Detection Kit | |
---|---|
Intended use | Qualitative detection of COVID-19 |
Specimen type | RNA extracted in nasal swab or Sputum |
Target | RdRP, S |
Product contents |
3X RT MasterMix (Yellow) COVID-19 Primer & Probe Mixture (Brown) COVID-19 positive control (Red) RNase free water (Blue) COVID-19 Extraction Control (Red,Yellow sticker on top) |
LoD | 0.66 copies/㎕ |
Storage condition |
-15 ℃ ~ -25 ℃ |
* Simplified Procedure when make mixture
(Number of tubes minimized)!
KHMedical Ref | Product Name | Shelf Life |
RV008 | RADI COVID-19 Detection Kit | 8 months |
MP002 | RADI PREP Swab and Stool DNA/RNA Kit | 18 months |
To test the Sensitivity and Limit of Detection(LoD) of RADI COVID-19 Detection Kit, synthesized RNA by
in vitro transcription was diluted to 4 concentrations and ran 24 times for each.
LOD IS 0.66 COPIES/㎕
Target | Copies/㎕ | Mean Ct | Result in agreement |
Percent agreement |
S gene | 1.66 | 37.62 | 24/24 | 100% |
0.66 | 39.88 | 23/24 | 95.83% | |
0.5 | 40.18 | 24/24 | 100% | |
0.33 | 39.57 | 20/24 | 83.32% |
RdRP gene | Copies/㎕ | Mean Ct | Result in agreement |
Percent agreement |
S gene | 1.66 | 36.55 | 24/24 | 100% |
0.66 | 38.06 | 23/24 | 95.83% | |
0.5 | 39.08 | 24/24 | 91.66% | |
0.33 | 38.47 | 21/24 | 87.49% |
* IPC mean Ct : 22.93
RADI COVID-19 Detection KIT did not cross-react with any of 53 pathogens.
RADI COVID-19 Detection Kit’s Primer & Probe design could detect up to 10 copies/rxn compared to
WHO Primer & Probe which detect up to 10² copies/rxn for E gene and 10³ copies/rxn for RdRP gene
Copies / rxn | S gene | RdRP gene | ||
106 | 21.06 | 21.2 | 21.46 | 21.63 |
105 | 25.05 | 24.97 | 25.01 | 24.65 |
104 | 28.99 | 29.22 | 28.22 | 28.29 |
103 | 32.47 | 32.62 | 31.96 | 31.81 |
102 | 36.51 | 36.41 | 36.4 | 35.18 |
101 | 39.95 | 39.66 | 39.18 | 38.05 |
E gene | RdRP gene | ||
23.24 | 22.72 | 24.75 | 24.78 |
26.48 | 26.34 | 28.17 | 28.12 |
29.90 | 29.88 | 31.44 | 31.31 |
32.48 | 32.26 | 34.61 | 33.72 |
35.69 | 36.76 | N/A | N/A |
N/A | N/A | N/A | N/A |
S gene
RdRP gene
E gene
RdRP gene